Small Molecule · Itruvone

Itruvone

  • Fast Track

Itruvone (development code PH10) is a synthetic, odorless neuroactive 'pherine' (vomeropherine) nasal spray in development for major depressive disorder. Chemically a pregnane steroid (pregn-4-en-20-yn-3-one). Administered intranasally at microgram-level doses, it is proposed to act on chemosensory neurons in the nasal passages that modulate olfactory-amygdala neural circuits, without systemic absorption or entry into the brain. Originated by Pherin Pharmaceuticals and developed by Vistagen Therapeutics.

Also known as: Itruvone, PH10, PH-10, 21321-89-1, pregn-4-en-20-yn-3-one

Modality
Small molecule
Chemical class
neuroactive steroid, pregnane steroid
DEA schedule
Unscheduled
Chemistry
Single enantiomer
Highest phase
Phase 2
Designations
Fast Track

Mechanism of action

Itruvone is a synthetic neuroactive 'pherine' (vomeropherine) delivered as an intranasal microgram-dose spray. Its proposed rapid-onset mechanism is non-systemic: it engages and activates chemosensory neurons in the nasal passages that connect to olfactory-amygdala neural circuits, increasing activity of the limbic-hypothalamic sympathetic nervous system and catecholamine release to produce antidepressant effects. Preclinical biodistribution work found radiolabeled [14C]PH10 essentially undetectable in the brain, blood, and plasma after intranasal dosing, supporting a peripheral (nasal-neuron) site of action with no measurable systemic exposure. No characterized systemic molecular receptor target has been published; the nasal chemosensory receptor mediating its effect remains uncharacterized.

TargetActionAffinity
Nasal chemosensory neuron receptor (uncharacterized)primaryUnknown

Formulations

FormulationRouteRegimenPharmacokinetics
Itruvone intranasal spraynasal spray
Microgram-level intranasal doses; Phase 2A studied 3.2 µg and 6.4 µg once daily for 8 weeks
IntranasalOnce daily

Development timeline

Phase 2Feb 2020 – Jun 2023
  1. Favorable safety/tolerability results from U.S. Phase 1 bridging study (single-center, randomized, double-blind, placebo-controlled, healthy adults; first dosed Jan 2023, last patient last visit Mar 2023) announced; no serious adverse events or AE discontinuations. Enables planned U.S. Phase 2B development. Program remains Phase 2 overall.
  2. FDA granted Fast Track designation for development of PH10 (itruvone) to treat major depressive disorder.
  3. metPositive Phase 2A study of PH10 (itruvone) in MDD: statistically significant rapid-onset antidepressant effect on HAM-D-17 vs placebo

Itruvone for Major depressive disorder

Phase 2ActiveMajor depressive disorder indication →

Itruvone (PH10) for major depressive disorder. A positive randomized, double-blind, placebo-controlled Phase 2A study (HAM-D-17 endpoint) was conducted in Mexico and published; a U.S. Phase 1 bridging safety study in healthy adults completed in 2023 with favorable safety/tolerability, enabling planned U.S. Phase 2B development as a non-systemic, stand-alone MDD treatment. FDA Fast Track designation granted December 2022. As of 2025, staged for potential U.S. Phase 2B (Vistagen or a strategic partner).

Readouts

  • 2020-02-19ReportedFull resultsmet

    Positive Phase 2A study of PH10 (itruvone) in MDD: statistically significant rapid-onset antidepressant effect on HAM-D-17 vs placebo

Identifiers

Sources

  1. VistaGen Announces Positive Results of Newly Published Exploratory Phase 2a Study of PH10 for Rapid-Onset Treatment of Major Depressive Disorder — VistaGen Therapeutics / PR Newswire
  2. Vistagen Announces Results of Successful U.S. Phase 1 Study of Itruvone (PH10), Enabling U.S. Phase 2B Development for Treatment of Major Depressive Disorder — Vistagen Therapeutics / Business Wire
  3. Vistagen Receives FDA Fast Track Designation for Development of PH10 to Treat Major Depressive Disorder — Vistagen Therapeutics / Business Wire
  4. Vistagen Reports New Preclinical Data Supporting Itruvone (PH10) Nasal Spray — VistaGen Therapeutics
  5. Vistagen Reports New Preclinical Data Supporting Itruvone (PH10) Nasal Spray's Potential Antidepressant Activity via Peripheral Nasal Neurons without Entry into the Brain — Vistagen Therapeutics / Business Wire